Features
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ProtoCOL is equipped with a unique colony detection system which combines special LED illumination and digital color camera control with powerful software. ProtoCOL comes standard with built in computer to minimize the foot print on lab bench. Computer specifications are updated as new processors and other advanced components become available. ProtoCOL is available in two different models, ProtocCOL SR (standard resolution) or ProtoCOL HR (high resolution). Both systems come complete with a 19” flat screen monitor, keyboard and mouse. |
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ProtoCOL SR ProtoCOL software provides accurate analysis and enumeration of bacterial plates. The system is able to compensate for writing on plates, variations in agar thickness, touching colonies, meniscus effects, spreaders, bubbles, debris, different sized colonies and different colored media. Measuring applications include enumeration of pour, spread and spiral plates, multi well plate analysis and inhibition zone measurement. Typical measuring time is 1 second/ plate. For standard 90mm Petri dishes, the smallest detectable colony is >0.2mm. Higher magnifications are available with an external camera. Software allows user to automatically set up serial dilution. A series is established and read with the mean count automatically calculated and reported. Users are also provided with detailed colony size statistics. Instant size distribution histograms and tables provide immediate comparisons between plate populations and can be used to highlight colonies of specific sizes. Users can easily create their own size class intervals and store them for reuse. ProtoCOL software also allows users to draw exclude regions, exclude problem areas on a plate and separate touching colonies. ProtoCOL ensures no transcription errors as results are instantly transferred to a results table in a secured database. Different levels of users are governed by password control, ensuring compliance with quality procedures and GLP. Users who wish to reread previously analyzed samples using automatic or manual methods will automatically have a copy of the original result stored along with the new result. Users can configure ProtoCOL to request a “reason for change” free text comment which is recorded with the data in a comments field. Users can choose to save true color sample images for archive purposes; these images can be printed out directly from ProtoCOL software. One button click automatically transfers results to and Excel spreadsheet. |
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ProtoCOL HR ProtoCOL HR employs all the features and benefits of the standard ProtoCOL system with the added benefit of a high resolution camera. The HR version has 4 times the resolution of ProtoCOL SR. For standard 90mm Petri dishes, the smallest detectable colony is >0.1mm. Higher magnifications are available with an external camera. ProtoCOL HR is ideal for applications where the enumeration of micro colonies and / or fine measurement of inhibition zones are required. Typical applications for ProtoCOL HR include enumeration of water filtration samples, samples where typical colonies sizes are less than 100 microns and automated inhibition zone measuring (disk diffusion or drop method). |
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Literature
Product Documentation:
ProtoCOL FAQs (13.2 kB)
ProtoCOLBrochure (507.31 kB)
Protocol Network Instructions (78.77 kB)
Specs
| ProtoCOL SR | |
| Measurement time | 0.9 seconds |
| Resolution | 0.2mm (for standard 90mm Petri dishes) |
| Illumination | Upper incident light, bottom transmitted light and dark field using LED arrays |
| Measurement modes | Pour plate, spiral plate, inhibition zones, antibacterial susceptibility |
| Detection | Fully automatic with manual override |
| Count modes | Separation of touching colonies, shape discrimination, area exclusion, colony inclusion/exclusion |
| Dilution series | User definable |
| Results | Display, storage and printing of up to 20 data items for each sample |
| Image storage/Retrieval | Storage of >20,000 images on local hard disk |
| External connections | Mouse, keyboard, bar code reader, monitor, external camera, ethernet |
| Storage | Hard disk and floppy disk |
| Camera | Zero distortion color digital with variable shutter speed |
| Camera lens | 6mm high resolution f 1.2 - closed |
| Power input | 110V 50/60 hz |
| Weight | 15 Kg |
| Dimensions | 452mm X 380mm X 452mm |
| Included computer specifications | Windows XP Professional OS |
| min. 2.0Ghz processor | |
| min. 20Gb Hard drive | |
| USB connection | |
| 1200 X 1600 True Color Graphics display | |
| Floppy drive | |
| ProtoCOL HR | |
| Measurement time | 0.9 seconds |
| Resolution | 0.070mm |
| Illumination | Upper incident light, bottom transmitted light and dark field using LED arrays |
| Measurement modes | Pour plate, spiral plate, inhibition zones, antibacterial susceptibility |
| Detection | Fully automatic with manual override |
| Count modes | Separation of touching colonies, shape discrimination, area exclusion, colony inclusion/exclusion |
| Dilution series | User definable |
| Results | Display, storage and printing of up to 20 data items for each sample |
| Image storage/Retrieval | Storage of >20,000 images on local hard disk |
| External connections | Mouse, keyboard, bar code reader, monitor, external camera, ethernet |
| Storage | Hard disk and floppy disk |
| Camera | Zero distortion color digital with variable shutter speed |
| Camera lens | 8.5mm high resolution f 1.2 - closed |
| Power input | 110V 50/60 hz |
| Weight | 15 Kg |
| Dimensions | 452mm X 380mm X 452mm |
| Included computer specifications | Windows XP Professional OS |
| min. 2.0Ghz processor | |
| min. 20Gb Hard drive | |
| USB connection | |
| 1200 X 1600 True Color Graphics display | |
| Floppy drive | |
Accessories
Color differentiation software module:
Differentiates up to seven different colony types based on color. Useful with chromogenic media.
Standard resolution (SR) external camera with lens:
For counting larger or multi-well plates; allows for adjustable focal length, as opposed to the fixed focal length for 90mm plates that is standard for ProtoCOL.
High resolution (HR) external camera with lens:
4 times the resolution of the standard resolution external camera
Lighted macro stand:
For use with external camera. Allows viewing of large plates.
Provides lighting for external camera
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C – mount for external camera connection to microscope
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Customized plate holder
Requires sample blank Petri dish for manufacturing
Bar code reader
For instant reading and input of plate ID
Automated X - Y stage
For precise multi well analysis and enumeration
AutoZone automatic stage
For the analysis of large bioassay / multi-well plates
Protocol Darkroom (UV and White light Illumination):
The Protocol Darkroom option allows for direct enumeration and analysis of fluorescent entities (i.e. GFP assay). Benefits include, direct connection to the Protocol system, short and long wavelength UV trans/epi illumination and white light trans/epi illumination. This turnkey system offers an elegant solution to working with UV in a closed and safe environment. 
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