Features

BBL™ CHROMagar™ Orientation

BBL™ CHROMagar™ Orientation medium is a nonselective differentiated medium for the isolation, of urinary tract pathogens Escherichia coli, Enterococcus, Klebsiella-Enterobacter-Serratia (KES), Proteus-Morganella-Providencia (PMP).

Enjoy the following features and benefits with BBL™ CHROMagar™ Orientation:

• AOAC-RI approved
• Used as primary plate
• Confirmatory detection E. coli & Enterococcus.
• Time to results 24 hours
• E.coli Sensitivity 96.9.%, Specificity 98.9%
• Enterococcus Sensitivity 98.5% Specificity 97%

Results

After proper incubation, the plates should show isolated colonies. Enterobacter cloacae appear dark blue to medium blue colonies, Enterococcus faecalis appear as blue-green colonies, E.coli appear dark rose to pink with or without halos, Proteus mirabilis appear beige to brown colonies, Staph. aureus appear white to cream color colonies, and Strep. Agalactiae appear light blue green to light blue colonies with or without halos.

Principles
Some of the organisms encountered in UTIs produce enzymes either for the metabolism of lactose or glucosides or both. Other organisms produce none of these enzymes. For example, E. coli contains enzymes for lactose metabolism but is ß-glucosidase negative. Some members of the family Enterobacteriaceae are ß-glucosidase positive but do not contain enzymes necessary for lactose fermentation; others may contain both types of enzymes or none of them. ß-glucosidases are also found in gram-positive cocci, such as S. agalactiae and the enterococci. Tryptophan deaminase (TDA) is an enzyme characteristically found in the Proteus-Morganella-Providencia group.

Procedure

1. Inoculate CHROMagar^TM Orientation plate with a dilution of the specimen.

2. Incubate plates aerobically at 35’C for not less than 20 to 24 hours.

3. Observe plates for characteristic colonial morphology and color at 20-24 hours.

AOAC is a trademark and Performance Tested Methods Program is a service mark of AOAC International. CHROMagar is a trademark of Dr. A. Rambach. BD, BD Logo and BBL are trademarks of Becton, Dickinson and Company. ©2005 BD

Specs